2019年12月，贵州省东说念主民病院胸外科(Department of Thoracic Surgery, Guizhou Provincial People’s Hospital, Guiyang, Guizhou Province, China) Di Liu浑朴征询团队在《Journal of International Medical Research》上发表论文：

“Knockdown of RAD54B expression reduces cell proliferation and induces apoptosis in lung cancer cells”

“下调RAD54B抒发可箝制肺癌细胞增殖，教唆细胞凋一火”

Abstract：

Objective: RAD54 homolog B (RAD54B), a member of the SNF2/SWI2 superfamily, is implicated in homologous recombination, and high RAD54B expression predicts the prognostic outcomes of lung adenocarcinoma. However, its role in lung carcinogenesis was unclear so this was determined in the present study.

Methods: We evaluated the gene and protein expression of RAD54B in 15 lung adenocarcinoma tissues and matched adjacent healthy lung tissues by real-time PCR, immunohistochemical staining, and western blotting. A549 lung cancer cells were transduced with lentivirus carrying small hairpin RNA (shRNA) against RAD54B (shRAD54B) or control shRNA (shCtrl), and cell proliferation, viability, apoptosis, and caspase 3/7 activity were evaluated.

Results: RAD54B protein expression was significantly higher in lung adenocarcinoma tissues than in healthy lung tissues. RAD54B gene expression was high in A549 cells but was efficiently knocked down using shRAD54B with an infection efficiency of 80% and a knockdown ratio of 72.2% compared with shCtrl. Suppressing RAD54B expression in A549 cells significantly reduced cell proliferation and caspase 3/7 activity, and significantly increased the apoptotic rate.

Conclusions: RAD54B exerts an oncogenic role in lung cancer cell proliferation.

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标的:SNF2/SWI2超家眷成员RAD54同源B (RAD54B)参与同源重组，RAD54B的高抒发可瞻望肺腺癌的预后。相关词，其在肺癌发生中的作用尚不明晰，因此本征询详情了这少量。

神色:选拔及时荧光定量PCR、免疫组化染色和western blotting检测RAD54B在15例肺腺癌组织及匹配的掌握健康肺组织中的基因和卵白抒发。用佩带小发卡RNA (shRNA)的慢病毒将A549肺癌细胞转导至RAD54B (shRAD54B)或对照shRNA (shCtrl)，不雅察细胞增殖、活力、凋一火和caspase 3/7活性。

为止:RAD54B卵白在肺腺癌组织中的抒发显著高于宽泛肺组织。RAD54B基因在A549细胞中抒发量较高，但与shCtrl比拟，shRAD54B可有用敲除，感染恶果为80%，敲除率为72.2%。箝制RAD54B在A549细胞中的抒发可显耀裁汰细胞增殖和caspase 3/7活性，并显耀擢升细胞凋一火率。

论断:RAD54B在肺癌细胞增殖中具有致瘤作用。

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